Marchais et al. 2018 (PRJNA389737)
General Details
| Title | Exhaustive profiling in Arabidopsis reveals abundant polysome-associated 24-nt small RNAs including hitherto undescribed AGO5-associated pseudogene-derived siRNAs (psiRNAs) [sRNA] |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2017/06/08 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP108862 |
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| ENA | SRP108862 |
| GEO | |
| BioProject | PRJNA389737 |
Publication
| Title | |
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| Authors | Marchais A,Chevalier C,Voinnet O |
| Journal | RNA (New York, N.Y.) |
| Publication Date | 2019 Sep |
| Abstract | In a reductionist perspective, plant silencing small (s)RNAs are often classified as mediating nuclear transcriptional gene silencing (TGS) or cytosolic posttranscriptional gene silencing (PTGS). Among the PTGS diagnostics is the association of AGOs and their sRNA cargos with the translation apparatus. In Arabidopsis , this is observed for AGO1 loaded with micro(mi)RNAs and, accordingly, translational-repression (TR) is one layer of plant miRNA action. Using AGO1:miRNA-mediated TR as a paradigm, we explored, with two unrelated polysome-isolation methods, which, among the ten Arabidopsis AGOs and numerous sRNA classes, interact with translation. We found that representatives of all three AGO-clades associate with polysomes, including the TGS-effector AGO4 and stereotypical 24-nt sRNAs that normally mediate TGS of transposons/repeats. Strikingly, approximately half of these annotated 24-nt siRNAs displayed unique matches in coding regions/introns of genes, and in pseudogenes, but not in transposons/repeats commonly found in their vicinity. Protein-coding gene-derived 24-nt sRNAs correlate with gene-body methylation. Those derived from pseudogenes belong to two main clusters defined by their parental-gene expression patterns, and are vastly enriched in AGO5, itself found on polysomes. Based on their tight expression pattern in developing and mature siliques, their biogenesis, and genomic/epigenomic features of their loci-of-origin, we discuss potential roles for these hitherto unknown polysome-enriched, pseudogene-derived siRNAs. © 2019 Marchais et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. |
| PMC | PMC6800511 |
| PMID | 31138671 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR5660304 | PRJNA389737 | Arabidopsis thaliana | Ribo-Seq |  |
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| SRR5660305 | PRJNA389737 | Arabidopsis thaliana | Ribo-Seq | |
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| SRR5660306 | PRJNA389737 | Arabidopsis thaliana | Ribo-Seq | |
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| SRR5660307 | PRJNA389737 | Arabidopsis thaliana | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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