Solomon et al. 2017 (PRJNA391171)

Title	RNA editing by ADAR1 leads to context-dependent transcriptome-wide changes in RNA Secondary Structure
Organism
Number of Samples	4
Release Date	2017/06/19 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz

Title
Authors	Solomon O,Di Segni A,Cesarkas K,Porath HT,Marcu-Malina V,Mizrahi O,Stern-Ginossar N,Kol N,Farage-Barhom S,Glick-Saar E,Lerenthal Y,Levanon EY,Amariglio N,Unger R,Goldstein I,Eyal E,Rechavi G
Journal	Nature communications
Publication Date	2017 Nov 13
Abstract	Adenosine deaminase acting on RNA 1 (ADAR1) is the master RNA editor, catalyzing the deamination of adenosine to inosine. RNA editing is vital for preventing abnormal activation of cytosolic nucleic acid sensing pathways by self-double-stranded RNAs. Here we determine, by parallel analysis of RNA secondary structure sequencing (PARS-seq), the global RNA secondary structure changes in ADAR1 deficient cells. Surprisingly, ADAR1 silencing resulted in a lower global double-stranded to single-stranded RNA ratio, suggesting that A-to-I editing can stabilize a large subset of imperfect RNA duplexes. The duplexes destabilized by editing are composed of vastly complementary inverted Alus found in untranslated regions of genes performing vital biological processes, including housekeeping functions and type-I interferon responses. They are predominantly cytoplasmic and generally demonstrate higher ribosomal occupancy. Our findings imply that the editing effect on RNA secondary structure is context dependent and underline the intricate regulatory role of ADAR1 on global RNA secondary structure.
PMC	PMC5682290
PMID	29129909
DOI

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