Li et al. 2017 (PRJNA396456)

General Details

Title Base-resolution mapping reveals distinct classes of N1-methyladenosine methylome in nuclear- and mitochondrial-encoded transcripts
Organism
Number of Samples 1
Release Date 2017/07/30 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP114321
ENA SRP114321
GEO GSE102040
BioProject PRJNA396456

Publication

Title
Authors Li X, Xiong X, Zhang M, Wang K, Chen Y, Zhou J, Mao Y, Lv J, Yi D, Chen XW, Wang C, Qian SB, Yi C
Journal Molecular cell
Publication Date 2017 Dec 7
Abstract Gene expression can be post-transcriptionally regulated via dynamic and reversible RNA modifications. N 1 -methyladenosine (m 1 A) is a recently identified mRNA modification; however, little is known about its precise location and biogenesis. Here, we develop a base-resolution m 1 A profiling method, based on m 1 A-induced misincorporation during reverse transcription, and report distinct classes of m 1 A methylome in the human transcriptome. m 1 A in 5' UTR, particularly those at the mRNA cap, associate with increased translation efficiency. A different, small subset of m 1 A exhibit a GUUCRA tRNA-like motif, are evenly distributed in the transcriptome, and are dependent on the methyltransferase TRMT6/61A. Additionally, we show that m 1 A is prevalent in the mitochondrial-encoded transcripts. Manipulation of m 1 A level via TRMT61B, a mitochondria-localizing m 1 A methyltransferase, demonstrates that m 1 A in mitochondrial mRNA interferes with translation. Collectively, our approaches reveal distinct classes of m 1 A methylome and provide a resource for functional studies of m 1 A-mediated epitranscriptomic regulation. Copyright © 2017 Elsevier Inc. All rights reserved.
PMC PMC5722686
PMID 29107537
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR5882586 PRJNA396456 Homo sapiens HEK293 Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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