Jakobsson et al. 2018 (PRJNA408071)
General Details
| Title | The dual methyltransferase METTL13 targets N-terminus and Lys55 of eEF1A and regulates codon-specific translation rates |
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| Organism | |
| Number of Samples | 5 |
| Release Date | 2017/09/20 00:00 |
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Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP118278 |
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| ENA | SRP118278 |
| GEO | GSE104033 |
| BioProject | PRJNA408071 |
Publication
| Title | |
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| Authors | Jakobsson ME,Małecki JM,Halabelian L,Nilges BS,Pinto R,Kudithipudi S,Munk S,Davydova E,Zuhairi FR,Arrowsmith CH,Jeltsch A,Leidel SA,Olsen JV,Falnes PØ |
| Journal | Nature communications |
| Publication Date | 2018 Aug 24 |
| Abstract | Eukaryotic elongation factor 1 alpha (eEF1A) delivers aminoacyl-tRNA to the ribosome and thereby plays a key role in protein synthesis. Human eEF1A is subject to extensive post-translational methylation, but several of the responsible enzymes remain unknown. Using a wide range of experimental approaches, we here show that human methyltransferase (MTase)-like protein 13 (METTL13) contains two distinct MTase domains targeting the N terminus and Lys55 of eEF1A, respectively. Our biochemical and structural analyses provide detailed mechanistic insights into recognition of the eEF1A N terminus by METTL13. Moreover, through ribosome profiling, we demonstrate that loss of METTL13 function alters translation dynamics and results in changed translation rates of specific codons. In summary, we here unravel the function of a human MTase, showing that it methylates eEF1A and modulates mRNA translation in a codon-specific manner. |
| PMC | PMC6109062 |
| PMID | 30143613 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR6053327 | PRJNA408071 | Homo sapiens | HAP1 | Ribo-Seq | Cycloheximide |  |
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| SRR6053328 | PRJNA408071 | Homo sapiens | HAP1 | Ribo-Seq | Cycloheximide | |
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| SRR6053329 | PRJNA408071 | Homo sapiens | HAP1 | Ribo-Seq | Cycloheximide | |
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| SRR6053333 | PRJNA408071 | Homo sapiens | HAP1 | Ribo-Seq | Cycloheximide | |
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| SRR6053334 | PRJNA408071 | Homo sapiens | HAP1 | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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