Kuppers et al. 2019 (PRJNA415390)

General Details

Title N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis[Ribosome Profiling]
Organism
Number of Samples 6
Release Date 2017/10/23 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP120964
ENA SRP120964
GEO GSE105782
BioProject PRJNA415390

Publication

Title
Authors Kuppers DA,Arora S,Lim Y,Lim AR,Carter LM,Corrin PD,Plaisier CL,Basom R,Delrow JJ,Wang S,Hansen He H,Torok-Storb B,Hsieh AC,Paddison PJ
Journal Nature communications
Publication Date 2019 Oct 10
Abstract Many of the regulatory features governing erythrocyte specification, maturation, and associated disorders remain enigmatic. To identify new regulators of erythropoiesis, we utilize a functional genomic screen for genes affecting expression of the erythroid marker CD235a/GYPA. Among validating hits are genes coding for the N 6 -methyladenosine (m 6 A) mRNA methyltransferase (MTase) complex, including, METTL14, METTL3, and WTAP. We demonstrate that m 6 A MTase activity promotes erythroid gene expression programs through selective translation of ~300 m 6 A marked mRNAs, including those coding for SETD histone methyltransferases, ribosomal components, and polyA RNA binding proteins. Remarkably, loss of m 6 A marks results in dramatic loss of H3K4me3 marks across key erythroid-specific KLF1 transcriptional targets (e.g., Heme biosynthesis genes). Further, each m 6 A MTase subunit and a subset of their mRNAs targets are required for human erythroid specification in primary bone-marrow derived progenitors. Thus, m 6 A mRNA marks promote the translation of a network of genes required for human erythropoiesis.
PMC PMC6787028
PMID 31601799
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR6202813 PRJNA415390 Homo sapiens HEL cells Ribo-Seq Cycloheximide
SRR6202814 PRJNA415390 Homo sapiens HEL cells Ribo-Seq Cycloheximide
SRR6202815 PRJNA415390 Homo sapiens HEL cells Ribo-Seq Cycloheximide
SRR6202819 PRJNA415390 Homo sapiens HEL cells Ribo-Seq Cycloheximide
SRR6202820 PRJNA415390 Homo sapiens HEL cells Ribo-Seq Cycloheximide
SRR6202821 PRJNA415390 Homo sapiens HEL cells Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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