Kuppers et al. 2019 (PRJNA415390)
General Details
Title | N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis[Ribosome Profiling] |
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Organism | |
Number of Samples | 6 |
Release Date | 2017/10/23 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP120964 |
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ENA | SRP120964 |
GEO | GSE105782 |
BioProject | PRJNA415390 |
Publication
Title | |
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Authors | Kuppers DA,Arora S,Lim Y,Lim AR,Carter LM,Corrin PD,Plaisier CL,Basom R,Delrow JJ,Wang S,Hansen He H,Torok-Storb B,Hsieh AC,Paddison PJ |
Journal | Nature communications |
Publication Date | 2019 Oct 10 |
Abstract | Many of the regulatory features governing erythrocyte specification, maturation, and associated disorders remain enigmatic. To identify new regulators of erythropoiesis, we utilize a functional genomic screen for genes affecting expression of the erythroid marker CD235a/GYPA. Among validating hits are genes coding for the N 6 -methyladenosine (m 6 A) mRNA methyltransferase (MTase) complex, including, METTL14, METTL3, and WTAP. We demonstrate that m 6 A MTase activity promotes erythroid gene expression programs through selective translation of ~300 m 6 A marked mRNAs, including those coding for SETD histone methyltransferases, ribosomal components, and polyA RNA binding proteins. Remarkably, loss of m 6 A marks results in dramatic loss of H3K4me3 marks across key erythroid-specific KLF1 transcriptional targets (e.g., Heme biosynthesis genes). Further, each m 6 A MTase subunit and a subset of their mRNAs targets are required for human erythroid specification in primary bone-marrow derived progenitors. Thus, m 6 A mRNA marks promote the translation of a network of genes required for human erythropoiesis. |
PMC | PMC6787028 |
PMID | 31601799 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR6202813 | PRJNA415390 | Homo sapiens | HEL cells | Ribo-Seq | Cycloheximide | ||||||||
SRR6202814 | PRJNA415390 | Homo sapiens | HEL cells | Ribo-Seq | Cycloheximide | ||||||||
SRR6202815 | PRJNA415390 | Homo sapiens | HEL cells | Ribo-Seq | Cycloheximide | ||||||||
SRR6202819 | PRJNA415390 | Homo sapiens | HEL cells | Ribo-Seq | Cycloheximide | ||||||||
SRR6202820 | PRJNA415390 | Homo sapiens | HEL cells | Ribo-Seq | Cycloheximide | ||||||||
SRR6202821 | PRJNA415390 | Homo sapiens | HEL cells | Ribo-Seq | Cycloheximide | ||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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