Datasets

Title	RPL12/uL11 phosphorylation regulates translation during mitosis [RIP-seq]
Organism
Number of Samples	12
Release Date	2018/03/21 00:00
Sequencing Types
Protocol Details

Title
Authors	Imami K,Milek M,Bogdanow B,Yasuda T,Kastelic N,Zauber H,Ishihama Y,Landthaler M,Selbach M
Journal	Molecular cell
Publication Date	2018 Oct 4
Abstract	Emerging evidence indicates that heterogeneity in ribosome composition can give rise to specialized functions. Until now, research mainly focused on differences in core ribosomal proteins and associated factors. The effect of posttranslational modifications has not been studied systematically. Analyzing ribosome heterogeneity is challenging because individual proteins can be part of different subcomplexes (40S, 60S, 80S, and polysomes). Here we develop polysome proteome profiling to obtain unbiased proteomic maps across ribosomal subcomplexes. Our method combines extensive fractionation by sucrose gradient centrifugation with quantitative mass spectrometry. The high resolution of the profiles allows us to assign proteins to specific subcomplexes. Phosphoproteomics on the fractions reveals that phosphorylation of serine 38 in RPL12/uL11, a known mitotic CDK1 substrate, is strongly depleted in polysomes. Follow-up experiments confirm that RPL12/uL11 phosphorylation regulates the translation of specific subsets of mRNAs during mitosis. Together, our results show that posttranslational modification of ribosomal proteins can regulate translation. Copyright © 2018 Elsevier Inc. All rights reserved.
PMC
PMID	30220558
DOI

Imami et al. 2019 (PRJNA445182)