Gerashchenko et al. 2020 (PRJNA445295)

General Details

Title Translation elongation rate varies among organs and decreases with age
Organism
Number of Samples 98
Release Date 2018/03/22 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP136268
ENA SRP136268
GEO GSE112223
BioProject PRJNA445295

Publication

Title
Authors Gerashchenko MV, Peterfi Z, Yim SH, Gladyshev VN
Journal Nucleic acids research
Publication Date 2021 Jan 25
Abstract There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to monitor and manipulate processes within organs and individual cells. It this study, we developed a labeling-free method for measuring organ- and cell-type-specific translation elongation rates in vivo. It is based on time-resolved delivery of translation initiation and elongation inhibitors in live animals followed by ribosome profiling. It also reports translation initiation sites in an organ-specific manner. Using this method, we found that the elongation rates differ more than 50% among mouse organs and determined them to be 6.8, 5.0 and 4.3 amino acids per second for liver, kidney, and skeletal muscle, respectively. We further found that the elongation rate is reduced by 20% between young adulthood and mid-life. Thus, translation, a major metabolic process in cells, is tightly regulated at the level of elongation of nascent polypeptide chains. © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC7826258
PMID 33264395
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR12671761 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671762 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671763 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671764 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671765 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671766 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671767 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671768 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671769 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671770 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671771 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR12671772 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880571 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880572 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880573 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880574 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880575 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880576 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880577 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880578 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880579 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880580 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880581 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880582 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880583 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880584 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880585 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880586 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880587 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880588 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880589 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880590 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880591 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880592 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880593 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880594 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880595 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880596 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880597 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880598 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880599 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880600 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880601 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880602 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880603 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880604 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880605 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880606 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880607 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880608 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880609 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880610 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880611 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880612 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880613 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880614 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880615 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880616 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880617 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880618 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880619 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880620 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880621 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880622 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880623 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880624 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880625 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880626 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880627 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880628 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880629 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880630 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880631 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880632 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880633 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880634 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880635 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880636 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880637 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880638 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880639 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880640 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880641 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880642 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880643 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880644 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880645 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880646 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880647 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880648 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880649 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880650 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880651 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880652 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880653 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880654 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880655 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
SRR6880656 PRJNA445295 Mus musculus 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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