Datasets

Title	Regulatory Discrimination of mRNAs by FMRP Controls Adult Neural Stem Cell Differentiation
Organism
Number of Samples	8
Release Date	2018/03/29 00:00
Sequencing Types
Protocol Details

Title
Authors	Liu B, Li Y, Stackpole EE, Novak A, Gao Y, Zhao Y, Zhao X, Richter JD
Journal	Proceedings of the National Academy of Sciences of the United States of America
Publication Date	2018 Nov 27
Abstract	Fragile X syndrome (FXS) is caused by the loss of fragile X mental retardation protein (FMRP), an RNA binding protein whose deficiency impacts many brain functions, including differentiation of adult neural stem cells (aNSCs). However, the mechanism by which FMRP influences these processes remains unclear. Here, we performed ribosome profiling and transcriptomic analysis of aNSCs in parallel from wild-type and Fmr1 knockout mice. Our data revealed diverse gene expression changes at both mRNA and translation levels. Many mitosis and neurogenesis genes were dysregulated primarily at the mRNA level, while numerous synaptic genes were mostly dysregulated at the translation level. Translational 'buffering', whereby changes in ribosome association with mRNA are compensated by alterations in RNA abundance, was also evident. Knockdown of NECDIN, an FMRP-repressed transcriptional factor, rescued neuronal differentiation. In addition, we discovered that FMRP regulates mitochondrial mRNA expression and energy homeostasis. Thus, FMRP controls diverse transcriptional and posttranscriptional gene expression programs critical for neural differentiation.
PMC	PMC6275535
PMID	30373821
DOI

Liu et al. 2018 (PRJNA448005)