Zou et al. 2019 (PRJNA448763)
General Details
| Title | Landscapes of gene translation in hepatocellular carcinoma tumors revealed by ribosome profiling |
|---|---|
| Organism | |
| Number of Samples | 20 |
| Release Date | 2018/04/04 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
|---|---|
Repository Details
| SRA | SRP137150 |
|---|---|
| ENA | SRP137150 |
| GEO | GSE112705 |
| BioProject | PRJNA448763 |
Publication
| Title | |
|---|---|
| Authors | Zou Q,Xiao Z,Huang R,Wang X,Wang X,Zhao H,Yang X |
| Journal | Theranostics |
| Publication Date | 2019 |
| Abstract | Despite the critical position of translation in the multilevel gene expression regulation program, high-resolution and genome-wide view of the landscape of RNA translation in solid tumors is still limited. Methods : With a ribosome profiling procedure optimized for solid tissue samples, we profiled the translatomes of liver tumors and their adjacent noncancerous normal liver tissues from 10 patients with hepatocellular carcinoma (HCC). A set of bioinformatics tools was then applied to these data for the mining of novel insights into the translation shifts in HCC. Results : This is the first translatome data resource for dissecting dysregulated translation in HCC at the sub-codon resolution. Based on our data, quantitative comparisons of mRNA translation rates yielded the genes and processes that were subjected to patient specific or universal dysregulations of translation efficiencies in tumors. For example, multiple proteins involved in extracellular matrix organization exhibited significant translational upregulation in tumors. We then experimentally validated the tumor-promoting functions of two such genes as examples: AGRN and VWA1. In addition, the data was also used for de novo annotation of the translatomes in tumors and normal tissues, including multiple types of novel non-canonical small ORFs, which would be a resource for further functional studies. Conclusions : The present study generates the first survey of the HCC translatome with ribosome profiling, which is an insightful data resource for dissecting the translatome shift in liver cancer, at sub-codon resolution. |
| PMC | PMC6592166 |
| PMID | 31281537 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SRR6939924 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide |  |
 |
 |
 |
|
|
|||
| SRR6939926 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939928 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939930 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939932 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939934 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939936 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939938 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939940 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939942 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939944 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939946 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939948 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939950 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939952 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939955 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939957 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939959 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939961 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| SRR6939963 | PRJNA448763 | Homo sapiens | Ribo-Seq | Cycloheximide | |
|
|
|
|
|
|||
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
ⓘ For more Information on the columns shown here see: About