Datasets

Title	DDX6 decouples translational repression from RNA degradation of miRNA targets [Ribosome Profiling]
Organism
Number of Samples	6
Release Date	2018/04/05 00:00
Sequencing Types
Protocol Details

Title
Authors	Freimer JW,Hu TJ,Blelloch R
Journal	eLife
Publication Date	2018 Jul 25
Abstract	Translation and mRNA degradation are intimately connected, yet the mechanisms that link them are not fully understood. Here, we studied these mechanisms in embryonic stem cells (ESCs). Transcripts showed a wide range of stabilities, which correlated with their relative translation levels and that did not change during early ESC differentiation. The protein DHH1 links translation to mRNA stability in yeast; however, loss of the mammalian homolog, DDX6, in ESCs did not disrupt the correlation across transcripts. Instead, the loss of DDX6 led to upregulated translation of microRNA targets, without concurrent changes in mRNA stability. The Ddx6 knockout cells were phenotypically and molecularly similar to cells lacking all microRNAs ( Dgcr8 knockout ESCs). These data show that the loss of DDX6 can separate the two canonical functions of microRNAs: translational repression and transcript destabilization. Furthermore, these data uncover a central role for translational repression independent of transcript destabilization in defining the downstream consequences of microRNA loss. © 2018, Freimer et al.
PMC	PMC6086665
PMID	30044225
DOI

Freimer et al. 2018 (PRJNA448921)