Engel et al. 2018 (PRJNA453992)
General Details
| Title | Ribosome profiling Seq of mouse adult cortex after acute stress |
|---|---|
| Organism | |
| Number of Samples | 12 |
| Release Date | 2018/04/27 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
|---|---|
Repository Details
| SRA | SRP143492 |
|---|---|
| ENA | SRP143492 |
| GEO | |
| BioProject | PRJNA453992 |
Publication
| Title | |
|---|---|
| Authors | Engel M,Eggert C,Kaplick PM,Eder M,Röh S,Tietze L,Namendorf C,Arloth J,Weber P,Rex-Haffner M,Geula S,Jakovcevski M,Hanna JH,Leshkowitz D,Uhr M,Wotjak CT,Schmidt MV,Deussing JM,Binder EB,Chen A |
| Journal | Neuron |
| Publication Date | 2018 Jul 25 |
| Abstract | N 6 -methyladenosine (m 6 A) and N 6 ,2'-O-dimethyladenosine (m 6 Am) are abundant mRNA modifications that regulate transcript processing and translation. The role of both, here termed m 6 A/m, in the stress response in the adult brain in vivo is currently unknown. Here, we provide a detailed analysis of the stress epitranscriptome using m 6 A/m-seq, global and gene-specific m 6 A/m measurements. We show that stress exposure and glucocorticoids region and time specifically alter m 6 A/m and its regulatory network. We demonstrate that deletion of the methyltransferase Mettl3 or the demethylase Fto in adult neurons alters the m 6 A/m epitranscriptome, increases fear memory, and changes the transcriptome response to fear and synaptic plasticity. Moreover, we report that regulation of m 6 A/m is impaired in major depressive disorder patients following glucocorticoid stimulation. Our findings indicate that brain m 6 A/m represents a novel layer of complexity in gene expression regulation after stress and that dysregulation of the m 6 A/m response may contribute to the pathophysiology of stress-related psychiatric disorders. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved. |
| PMC | PMC6069762 |
| PMID | 30048615 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SRR7074420 | PRJNA453992 | Mus musculus | Ribo-Seq |  |
 |
 |
 |
|
|
||||
| SRR7074421 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074422 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074423 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074424 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074425 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074426 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074427 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074428 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074429 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074430 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| SRR7074431 | PRJNA453992 | Mus musculus | Ribo-Seq | |
|
|
|
|
|
||||
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
ⓘ For more Information on the columns shown here see: About