Sapkota et al. 2019 (PRJNA475144)

Title	Cell type specific profiling of alternative translation identifies novel protein isoforms in the mouse brain
Organism
Number of Samples	4
Release Date	2018/06/07 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz

Title
Authors	Sapkota D,Lake AM,Yang W,Yang C,Wesseling H,Guise A,Uncu C,Dalal JS,Kraft AW,Lee JM,Sands MS,Steen JA,Dougherty JD
Journal	Cell reports
Publication Date	2019 Jan 15
Abstract	Alternative translation initiation and stop codon readthrough in a few well-studied cases have been shown to allow the same transcript to generate multiple protein variants. Because the brain shows a particularly abundant use of alternative splicing, we sought to study alternative translation in CNS cells. We show that alternative translation is widespread and regulated across brain transcripts. In neural cultures, we identify alternative initiation on hundreds of transcripts, confirm several N-terminal protein variants, and show the modulation of the phenomenon by KCl stimulation. We also detect readthrough in cultures and show differential levels of normal and readthrough versions of AQP4 in gliotic diseases. Finally, we couple translating ribosome affinity purification to ribosome footprinting (TRAP-RF) for cell-type-specific analysis of neuronal and astrocytic translational readthrough in the mouse brain. We demonstrate that this unappreciated mechanism generates numerous and diverse protein isoforms in a cell-type-specific manner in the brain. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.
PMC	PMC6392083
PMID	30650354
DOI

ⓘ For more Information on the columns shown here see: About