Datasets

Title	Iron and Heme Coordinate Erythropoiesis through HRI-Mediated Regulation of Protein Translation and Gene Expression
Organism
Number of Samples	8
Release Date	2018/09/02 00:00
Sequencing Types
Protocol Details

Title
Authors	Zhang S, Macias-Garcia A, Ulirsch JC, Velazquez J, Butty VL, Levine SS, Sankaran VG, Chen JJ
Journal	eLife
Publication Date	2019 Apr 29
Abstract	Iron and heme play central roles in the production of red blood cells, but the underlying mechanisms remain incompletely understood. Heme-regulated eIF2α kinase (HRI) controls translation by phosphorylating eIF2α. Here, we investigate the global impact of iron, heme, and HRI on protein translation in vivo in murine primary erythroblasts using ribosome profiling. We validate the known role of HRI-mediated translational stimulation of integratedstressresponse mRNAs during iron deficiency in vivo. Moreover, we find that the translation of mRNAs encoding cytosolic and mitochondrial ribosomal proteins is substantially repressed by HRI during iron deficiency, causing a decrease in cytosolic and mitochondrial protein synthesis. The absence of HRI during iron deficiency elicits a prominent cytoplasmic unfolded protein response and impairs mitochondrial respiration. Importantly, ATF4 target genes are activated during iron deficiency to maintain mitochondrial function and to enable erythroid differentiation. We further identify GRB10 as a previously unappreciated regulator of terminal erythropoiesis. © 2019, Zhang et al.
PMC	PMC6533081
PMID	31033440
DOI

Run Accession	Study Accession	Scientific Name	Cell Line	Library Type	Treatment	GWIPS-viz	Trips-Viz	Reads	BAM	BigWig (F)	BigWig (R)
SRR7777908	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777909	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777910	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777911	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777912	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777913	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777914	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
SRR7777915	PRJNA488955	Mus musculus	NA_Primary basophilic erythroblasts	Ribo-Seq	0.0
Run Accession	Study Accession	Scientific Name	Cell Line	Library Type	Treatment	GWIPS-viz	Trips-Viz	Reads	BAM	BigWig (F)	BigWig (R)

Zhang et al. 2018 (PRJNA488955)