Uttam et al. 2018 (PRJNA510323)

General Details

Title Translational profiling of dorsal root ganglia and spinal cord in a mouse model of neuropathic pain
Organism
Number of Samples 16
Release Date 2018/12/17 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP173586
ENA SRP173586
GEO GSE123919
BioProject PRJNA510323

Publication

Title
Authors Uttam S,Wong C,Amorim IS,Jafarnejad SM,Tansley SN,Yang J,Prager-Khoutorsky M,Mogil JS,Gkogkas CG,Khoutorsky A
Journal Neurobiology of pain (Cambridge, Mass.)
Publication Date 2018 Aug-Dec
Abstract Acute pain serves as a protective mechanism, guiding the organism away from actual or potential tissue injury. In contrast, chronic pain is a debilitating condition without any obvious physiological function. The transition to, and the maintenance of chronic pain require new gene expression to support biochemical and structural changes within the pain pathway. The regulation of gene expression at the level of mRNA translation has emerged as an important step in the control of protein expression in the cell. Recent studies show that signaling pathways upstream of mRNA translation, such as mTORC1 and ERK, are upregulated in chronic pain conditions, and their inhibition effectively alleviates pain in several animal models. Despite this progress, mRNAs whose translation is altered in chronic pain conditions remain largely unknown. Here, we performed genome-wide translational profiling of dorsal root ganglion (DRG) and spinal cord dorsal horn tissues in a mouse model of neuropathic pain, spared nerve injury (SNI), using the ribosome profiling technique. We identified distinct subsets of mRNAs that are differentially translated in response to nerve injury in both tissues. We discovered key converging upstream regulators and pathways linked to mRNA translational control and neuropathic pain. Our data are crucial for the understanding of mechanisms by which mRNA translation promotes persistent hypersensitivity after nerve injury.
PMC PMC6428075
PMID 30906902
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR8327789 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327790 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327793 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327794 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327797 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327798 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327801 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327802 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327805 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327806 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327809 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327810 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327813 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327814 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327817 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
SRR8327818 PRJNA510323 Mus musculus Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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