Anisimova et al. 2020 (PRJNA510416)

General Details

Title Age-resolved ribosome profiling and RNA-Seq data of mice liver and kidney samples
Organism
Number of Samples 28
Release Date 2018/12/17 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP173659
ENA SRP173659
GEO GSE123981
BioProject PRJNA510416

Publication

Title
Authors Anisimova AS, Meerson MB, Gerashchenko MV, Kulakovskiy IV, Dmitriev SE, Gladyshev VN
Journal Proceedings of the National Academy of Sciences of the United States of America
Publication Date 2020 Jul 7
Abstract Protein synthesis represents a major metabolic activity of the cell. However, how it is affected by aging and how this in turn impacts cell function remains largely unexplored. To address this question, herein we characterized age-related changes in both the transcriptome and translatome of mouse tissues over the entire life span. We showed that the transcriptome changes govern those in the translatome and are associated with altered expression of genes involved in inflammation, extracellular matrix, and lipid metabolism. We also identified genes that may serve as candidate biomarkers of aging. At the translational level, we uncovered sustained down-regulation of a set of 5'-terminal oligopyrimidine (5'-TOP) transcripts encoding protein synthesis and ribosome biogenesis machinery and regulated by the mTOR pathway. For many of them, ribosome occupancy dropped twofold or even more. Moreover, with age, ribosome coverage gradually decreased in the vicinity of start codons and increased near stop codons, revealing complex age-related changes in the translation process. Taken together, our results reveal systematic and multidimensional deregulation of protein synthesis, showing how this major cellular process declines with age.
PMC PMC7354943
PMID 32576685
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR11869332 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869322 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869324 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869326 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869328 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869330 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869334 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869336 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869338 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869340 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869342 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869344 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869346 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869348 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869350 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869352 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869354 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869356 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869358 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869360 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869362 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869364 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869366 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869368 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869370 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869372 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869374 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
SRR11869376 PRJNA510416 Mus musculus 0.0 Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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