Siang et al. 2019 (PRJNA511455)

General Details

Title The RNA-binding protein HuR is a negative regulator in adipogenesis [Ribo-seq_RNA-seq_matched]
Organism
Number of Samples 4
Release Date 2018/12/21 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP174218
ENA SRP174218
GEO GSE124279
BioProject PRJNA511455

Publication

Title
Authors Siang DTC,Lim YC,Kyaw AMM,Win KN,Chia SY,Degirmenci U,Hu X,Tan BC,Walet ACE,Sun L,Xu D
Journal Nature communications
Publication Date 2020 Jan 10
Abstract Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown and overexpression of HuR in primary adipocyte culture enhances and inhibits adipogenesis in vitro, respectively. Fat-specific knockout of HuR significantly enhances adipogenic gene program in adipose tissues, accompanied by a systemic glucose intolerance and insulin resistance. HuR knockout also results in depot-specific phenotypes: it can repress myogenesis program in brown fat, enhance inflammation program in epidydimal white fat and induce browning program in inguinal white fat. Mechanistically, HuR may inhibit adipogenesis by recognizing and modulating the stability of hundreds of adipocyte transcripts including Insig1, a negative regulator during adipogenesis. Taken together, our work establishes HuR as an important posttranscriptional regulator of adipogenesis and provides insights into how RNA processing contributes to adipocyte development.
PMC PMC6954112
PMID 31924774
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR8363858 PRJNA511455 Mus musculus Ribo-Seq
SRR8363859 PRJNA511455 Mus musculus Ribo-Seq
SRR8363860 PRJNA511455 Mus musculus
SRR8363861 PRJNA511455 Mus musculus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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