Song et al. 2019 (PRJNA512491)
General Details
| Title | Differential roles of human PUS10 in miRNA processing and tRNA pseudouridylation |
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| Organism | |
| Number of Samples | 33 |
| Release Date | 2019/01/02 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP175017 |
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| ENA | SRP175017 |
| GEO | GSE124558 |
| BioProject | PRJNA512491 |
Publication
| Title | |
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| Authors | Song J,Zhuang Y,Zhu C,Meng H,Lu B,Xie B,Peng J,Li M,Yi C |
| Journal | Nature chemical biology |
| Publication Date | 2020 Feb |
| Abstract | Pseudouridine synthases (PUSs) are responsible for installation of pseudouridine (Ψ) modification in RNA. However, the activity and function of the PUS enzymes remain largely unexplored. Here we focus on human PUS10 and find that it co-expresses with the microprocessor (DROSHA-DGCR8 complex). Depletion of PUS10 results in a marked reduction of the expression level of a large number of mature miRNAs and concomitant accumulation of unprocessed primary microRNAs (pri-miRNAs) in multiple human cells. Mechanistically, PUS10 directly binds to pri-miRNAs and interacts with the microprocessor to promote miRNA biogenesis. Unexpectedly, this process is independent of the catalytic activity of PUS10. Additionally, we develop a sequencing method to profile Ψ in the tRNAome and report PUS10-dependent Ψ sites in tRNA. Collectively, our findings reveal differential functions of PUS10 in nuclear miRNA processing and in cytoplasmic tRNA pseudouridylation. |
| PMC | |
| PMID | 31819270 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR8383359 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq |  |
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| SRR8383361 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR8383363 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR8383365 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR8383367 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855601 | PRJNA512491 | Homo sapiens | HEK293 | RNA-Seq | |
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| SRR9855602 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855603 | PRJNA512491 | Homo sapiens | HEK293 | RNA-Seq | |
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| SRR9855604 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855605 | PRJNA512491 | Homo sapiens | HEK293 | RNA-Seq | |
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| SRR9855606 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855607 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855608 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855609 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855610 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855611 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855612 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855613 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855614 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855615 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855616 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855617 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855618 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855619 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855620 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855621 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855622 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855623 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855624 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855625 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855626 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| SRR9855627 | PRJNA512491 | Homo sapiens | HEK293 | |
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| SRR9855628 | PRJNA512491 | Homo sapiens | HEK293 | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
ⓘ For more Information on the columns shown here see: About