Datasets

Title	Accurate annotation of human protein-coding small open reading frames
Organism
Number of Samples	16
Release Date	2019/01/16 00:00
Sequencing Types
Protocol Details

Title
Authors	Martinez TF,Chu Q,Donaldson C,Tan D,Shokhirev MN,Saghatelian A
Journal	Nature chemical biology
Publication Date	2020 Apr
Abstract	Functional protein-coding small open reading frames (smORFs) are emerging as an important class of genes. However, the number of translated smORFs in the human genome is unclear because proteogenomic methods are not sensitive enough, and, as we show, Ribo-seq strategies require additional measures to ensure comprehensive and accurate smORF annotation. Here, we integrate de novo transcriptome assembly and Ribo-seq into an improved workflow that overcomes obstacles with previous methods, to more confidently annotate thousands of smORFs. Evolutionary conservation analyses suggest that hundreds of smORF-encoded microproteins are likely functional. Additionally, many smORFs are regulated during fundamental biological processes, such as cell stress. Peptides derived from smORFs are also detectable on human leukocyte antigen complexes, revealing smORFs as a source of antigens. Thus, by including additional validation into our smORF annotation workflow, we accurately identify thousands of unannotated translated smORFs that will provide a rich pool of unexplored, functional human genes.
PMC	PMC7085969
PMID	31819274
DOI

Martinez et al. 2019 (PRJNA515538)