Chen et al. 2019 (PRJNA525595)
General Details
| Title | Selective translation by alternative bacterial ribosomes |
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| Organism | |
| Number of Samples | 12 |
| Release Date | 2019/03/05 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP187517 |
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| ENA | SRP187517 |
| GEO | |
| BioProject | PRJNA525595 |
Publication
| Title | |
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| Authors | Chen YX,Xu ZY,Ge X,Sanyal S,Lu ZJ,Javid B |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Publication Date | 2020 Aug 11 |
| Abstract | Alternative ribosome subunit proteins are prevalent in the genomes of diverse bacterial species, but their functional significance is controversial. Attempts to study microbial ribosomal heterogeneity have mostly relied on comparing wild-type strains with mutants in which subunits have been deleted, but this approach does not allow direct comparison of alternate ribosome isoforms isolated from identical cellular contexts. Here, by simultaneously purifying canonical and alternative RpsR ribosomes from Mycobacterium smegmatis , we show that alternative ribosomes have distinct translational features compared with their canonical counterparts. Both alternative and canonical ribosomes actively take part in protein synthesis, although they translate a subset of genes with differential efficiency as measured by ribosome profiling. We also show that alternative ribosomes have a relative defect in initiation complex formation. Furthermore, a strain of M. smegmatis in which the alternative ribosome protein operon is deleted grows poorly in iron-depleted medium, uncovering a role for alternative ribosomes in iron homeostasis. Our work confirms the distinct and nonredundant contribution of alternative bacterial ribosomes for adaptation to hostile environments. Copyright © 2020 the Author(s). Published by PNAS. |
| PMC | PMC7431078 |
| PMID | 32723820 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR8668645 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq |  |
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| SRR8668646 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668647 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668648 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668649 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668650 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668653 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668654 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668655 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668656 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668657 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| SRR8668658 | PRJNA525595 | Mycolicibacterium smegmatis | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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