Khalsa et al. 2019 (PRJNA527039)

General Details

Title Translatome and transcriptome analysis of resting B and T cells from mice
Organism
Number of Samples 4
Release Date 2019/03/14 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP188420
ENA SRP188420
GEO GSE128298
BioProject PRJNA527039

Publication

Title
Authors Khalsa JK, Chawla AS, Prabhu SB, Vats M, Dhar A, Dev G, Das N, Mukherjee S, Tanwar S, Banerjee H, Durdik JM, Bal V, George A, Rath S, Arimbasseri GA
Journal Immunology
Publication Date 2019 Oct
Abstract Activation of B and T lymphocytes leads to major remodelling of the metabolic landscape of the cells enabling their post-activation functions. However, naive B and T lymphocytes also show metabolic differences, and the genesis, nature and functional significance of these differences are not yet well understood. Here we show that resting B-cells appeared to have lower energy demands than resting T-cells as they consumed lower levels of glucose and fatty acids and produced less ATP. Resting B-cells are more dependent on OXPHOS, while T-cells show more dependence on aerobic glycolysis. However, despite an apparently higher energy demand, T lineage cells showed lower rates of protein synthesis than equivalent B lineage stages. These metabolic differences between the two lineages were established early during lineage differentiation, and were functionally significant. Higher levels of protein synthesis in B-cells were associated with increased synthesis of MHC class II molecules and other proteins associated with antigen internalization, transport and presentation. The combination of higher energy demand and lower protein synthesis in T-cells was consistent with their higher ATP-dependent motility. Our data provide an integrated perspective of the metabolic differences and their functional implications between the B and T lymphocyte lineages. © 2019 John Wiley & Sons Ltd.
PMC PMC6742768
PMID 31318442
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR8728404 PRJNA527039 Mus musculus NA_resting state B cells Ribo-Seq 0.0
SRR8728405 PRJNA527039 Mus musculus NA_resting state B cells Ribo-Seq 0.0
SRR8728406 PRJNA527039 Mus musculus NA_resting state T cells Ribo-Seq 0.0
SRR8728407 PRJNA527039 Mus musculus NA_resting state T cells Ribo-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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