Khalsa et al. 2019 (PRJNA527039)
General Details
| Title | Translatome and transcriptome analysis of resting B and T cells from mice |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2019/03/14 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP188420 |
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| ENA | SRP188420 |
| GEO | |
| BioProject | PRJNA527039 |
Publication
| Title | |
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| Authors | Khalsa JK,Chawla AS,Prabhu SB,Vats M,Dhar A,Dev G,Das N,Mukherjee S,Tanwar S,Banerjee H,Durdik JM,Bal V,George A,Rath S,Arimbasseri GA |
| Journal | Immunology |
| Publication Date | 2019 Oct |
| Abstract | Activation of B and T lymphocytes leads to major remodelling of the metabolic landscape of the cells enabling their post-activation functions. However, naive B and T lymphocytes also show metabolic differences, and the genesis, nature and functional significance of these differences are not yet well understood. Here we show that resting B-cells appeared to have lower energy demands than resting T-cells as they consumed lower levels of glucose and fatty acids and produced less ATP. Resting B-cells are more dependent on OXPHOS, while T-cells show more dependence on aerobic glycolysis. However, despite an apparently higher energy demand, T lineage cells showed lower rates of protein synthesis than equivalent B lineage stages. These metabolic differences between the two lineages were established early during lineage differentiation, and were functionally significant. Higher levels of protein synthesis in B-cells were associated with increased synthesis of MHC class II molecules and other proteins associated with antigen internalization, transport and presentation. The combination of higher energy demand and lower protein synthesis in T-cells was consistent with their higher ATP-dependent motility. Our data provide an integrated perspective of the metabolic differences and their functional implications between the B and T lymphocyte lineages. © 2019 John Wiley & Sons Ltd. |
| PMC | PMC6742768 |
| PMID | 31318442 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR8728404 | PRJNA527039 | Mus musculus | resting state B cells | Ribo-Seq |  |
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| SRR8728405 | PRJNA527039 | Mus musculus | resting state B cells | Ribo-Seq | |
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| SRR8728406 | PRJNA527039 | Mus musculus | resting state T cells | Ribo-Seq | |
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| SRR8728407 | PRJNA527039 | Mus musculus | resting state T cells | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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