Saini et al. 2019 (PRJNA533159)

General Details

Title Free circular introns with an unusual branchpoint in neuronal projections
Organism
Number of Samples 3
Release Date 2019/04/16 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP192787
ENA SRP192787
GEO
BioProject PRJNA533159

Publication

Title
Authors Saini H,Bicknell AA,Eddy SR,Moore MJ
Journal eLife
Publication Date 2019 Nov 7
Abstract The polarized structure of axons and dendrites in neuronal cells depends in part on RNA localization. Previous studies have looked at which polyadenylated RNAs are enriched in neuronal projections or at synapses, but less is known about the distribution of non-adenylated RNAs. By physically dissecting projections from cell bodies of primary rat hippocampal neurons and sequencing total RNA, we found an unexpected set of free circular introns with a non-canonical branchpoint enriched in neuronal projections. These introns appear to be tailless lariats that escape debranching. They lack ribosome occupancy, sequence conservation, and known localization signals, and their function, if any, is not known. Nonetheless, their enrichment in projections has important implications for our understanding of the mechanisms by which RNAs reach distal compartments of asymmetric cells. © 2019, Saini et al.
PMC PMC6879206
PMID 31697236
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR8919681 PRJNA533159 Rattus norvegicus Primary hippocampal neurons Ribo-Seq Cycloheximide
SRR8919682 PRJNA533159 Rattus norvegicus Primary hippocampal neurons Ribo-Seq Cycloheximide
SRR8919683 PRJNA533159 Rattus norvegicus Primary hippocampal neurons Ribo-Seq Cycloheximide
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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