Datasets

Title	Ribosome profiling of eIF3e-KD MCF-10a cells
Organism
Number of Samples	9
Release Date	2019/05/13 00:00
Sequencing Types
Protocol Details

Title
Authors	Lin Y,Li F,Huang L,Polte C,Duan H,Fang J,Sun L,Xing X,Tian G,Cheng Y,Ignatova Z,Yang X,Wolf DA
Journal	Molecular cell
Publication Date	2020 Aug 20
Abstract	eIF3, a multi-subunit complex with numerous functions in canonical translation initiation, is known to interact with 40S and 60S ribosomal proteins and translation elongation factors, but a direct involvement in translation elongation has never been demonstrated. We found that eIF3 deficiency reduced early ribosomal elongation speed between codons 25 and 75 on a set of ∼2,700 mRNAs encoding proteins associated with mitochondrial and membrane functions, resulting in defective synthesis of their encoded proteins. To promote elongation, eIF3 interacts with 80S ribosomes translating the first ∼60 codons and serves to recruit protein quality-control factors, functions required for normal mitochondrial physiology. Accordingly, eIF3e +/- mice accumulate defective mitochondria in skeletal muscle and show a progressive decline in muscle strength. Hence, eIF3 interacts with 80S ribosomes to enhance, at the level of early elongation, the synthesis of proteins with membrane-associated functions, an activity that is critical for mitochondrial physiology and muscle health. Copyright © 2020 Elsevier Inc. All rights reserved.
PMC
PMID	32589965
DOI

Lin et al. 2020 (PRJNA542601)