Buschauer et al. 2020 (PRJNA543493)

Title	The Ccr4-Not complex monitors the translating ribosome for codon optimality
Organism
Number of Samples	4
Release Date	2019/05/17 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz

Title
Authors	Buschauer R,Matsuo Y,Sugiyama T,Chen YH,Alhusaini N,Sweet T,Ikeuchi K,Cheng J,Matsuki Y,Nobuta R,Gilmozzi A,Berninghausen O,Tesina P,Becker T,Coller J,Inada T,Beckmann R
Journal	Science (New York, N.Y.)
Publication Date	2020 Apr 17
Abstract	Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in Saccharomyces cerevisiae This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMC	PMC8663607
PMID	32299921
DOI

ⓘ For more Information on the columns shown here see: About