Basak et al. 2019 (PRJNA545050)
General Details
| Title | Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A translation (Ribo-seq, RNA-seq) |
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| Organism | |
| Number of Samples | 16 |
| Release Date | 2019/05/28 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP199612 |
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| ENA | SRP199612 |
| GEO | GSE131809 |
| BioProject | PRJNA545050 |
Publication
| Title | |
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| Authors | Basak A,Munschauer M,Lareau CA,Montbleau KE,Ulirsch JC,Hartigan CR,Schenone M,Lian J,Wang Y,Huang Y,Wu X,Gehrke L,Rice CM,An X,Christou HA,Mohandas N,Carr SA,Chen JJ,Orkin SH,Lander ES,Sankaran VG |
| Journal | Nature genetics |
| Publication Date | 2020 Feb |
| Abstract | Increased production of fetal hemoglobin (HbF) can ameliorate the severity of sickle cell disease and β-thalassemia 1 . BCL11A represses the genes encoding HbF and regulates human hemoglobin switching through variation in its expression during development 2-7 . However, the mechanisms underlying the developmental expression of BCL11A remain mysterious. Here we show that BCL11A is regulated at the level of messenger RNA (mRNA) translation during human hematopoietic development. Despite decreased BCL11A protein synthesis earlier in development, BCL11A mRNA continues to be associated with ribosomes. Through unbiased genomic and proteomic analyses, we demonstrate that the RNA-binding protein LIN28B, which is developmentally expressed in a pattern reciprocal to that of BCL11A, directly interacts with ribosomes and BCL11A mRNA. Furthermore, we show that BCL11A mRNA translation is suppressed by LIN28B through direct interactions, independently of its role in regulating let-7 microRNAs, and that BCL11A is the major target of LIN28B-mediated HbF induction. Our results reveal a previously unappreciated mechanism underlying human hemoglobin switching that illuminates new therapeutic opportunities. |
| PMC | PMC7031047 |
| PMID | 31959994 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR9132358 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide |  |
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| SRR9132359 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132360 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132361 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132362 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132363 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132364 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132365 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132366 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132367 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132368 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132369 | PRJNA545050 | Homo sapiens | hematopoetic cells_adult erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132370 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132371 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132372 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| SRR9132373 | PRJNA545050 | Homo sapiens | hematopoetic cells_newborn erythroid cells | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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