Wallace et al. 2019 (PRJNA550038)
General Details
| Title | Ribosome profiling of Cryptococcus neoformans H99 |
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| Organism | |
| Number of Samples | 6 |
| Release Date | 2019/06/21 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP202195 |
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| ENA | SRP202195 |
| GEO | GSE133125 |
| BioProject | PRJNA550038 |
Publication
| Title | |
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| Authors | Wallace EWJ, Maufrais C, Sales-Lee J, Tuck LR, de Oliveira L, Feuerbach F, Moyrand F, Natarajan P, Madhani HD, Janbon G |
| Journal | Nucleic acids research |
| Publication Date | 2020 Mar 18 |
| Abstract | Eukaryotic protein synthesis generally initiates at a start codon defined by an AUG and its surrounding Kozak sequence context, but the quantitative importance of this context in different species is unclear. We tested this concept in two pathogenic Cryptococcus yeast species by genome-wide mapping of translation and of mRNA 5' and 3' ends. We observed thousands of AUG-initiated upstream open reading frames (uORFs) that are a major contributor to translation repression. uORF use depends on the Kozak sequence context of its start codon, and uORFs with strong contexts promote nonsense-mediated mRNA decay. Transcript leaders in Cryptococcus and other fungi are substantially longer and more AUG-dense than in Saccharomyces. Numerous Cryptococcus mRNAs encode predicted dual-localized proteins, including many aminoacyl-tRNA synthetases, in which a leaky AUG start codon is followed by a strong Kozak context in-frame AUG, separated by mitochondrial-targeting sequence. Analysis of other fungal species shows that such dual-localization is also predicted to be common in the ascomycete mould, Neurospora crassa. Kozak-controlled regulation is correlated with insertions in translational initiation factors in fidelity-determining regions that contact the initiator tRNA. Thus, start codon context is a signal that quantitatively programs both the expression and the structures of proteins in diverse fungi. © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. |
| PMC | PMC7049704 |
| PMID | 32020195 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR9336389 | PRJNA550038 | Cryptococcus neoformans | H9 | Ribo-Seq | 0.0 |  |
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| SRR9336391 | PRJNA550038 | Cryptococcus neoformans | HdGWO1 | Ribo-Seq | 0.0 | |
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| SRR9336393 | PRJNA550038 | Cryptococcus neoformans | H9 | Ribo-Seq | 0.0 | |
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| SRR9336395 | PRJNA550038 | Cryptococcus neoformans | HdAGO1 | Ribo-Seq | 0.0 | |
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| SRR9620586 | PRJNA550038 | Cryptococcus neoformans | JEC21 | Ribo-Seq | 0.0 | |
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| SRR9620588 | PRJNA550038 | Cryptococcus neoformans | JdAGO1 | Ribo-Seq | 0.0 | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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