Biever et al. 2020 (PRJNA550323)

General Details

Title Monosomes actively translate synaptic mRNAs in neuronal processes
Organism
Number of Samples 39
Release Date 2019/06/24 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP211959
ENA SRP211959
GEO
BioProject PRJNA550323

Publication

Title
Authors Biever A,Glock C,Tushev G,Ciirdaeva E,Dalmay T,Langer JD,Schuman EM
Journal Science (New York, N.Y.)
Publication Date 2020 Jan 31
Abstract To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMC
PMID 32001627
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR9596324 PRJNA550323 Mus musculus RNA-Seq
SRR9596323 PRJNA550323 Mus musculus RNA-Seq
SRR9596320 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596318 PRJNA550323 Mus musculus RNA-Seq
SRR9596317 PRJNA550323 Mus musculus RNA-Seq
SRR9596316 PRJNA550323 Mus musculus RNA-Seq
SRR9596315 PRJNA550323 Mus musculus RNA-Seq
SRR9596314 PRJNA550323 Mus musculus RNA-Seq
SRR9596313 PRJNA550323 Mus musculus RNA-Seq
SRR9596312 PRJNA550323 Mus musculus RNA-Seq
SRR9596311 PRJNA550323 Mus musculus RNA-Seq
SRR9596310 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596309 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596307 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596306 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596305 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596308 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596304 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596303 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596302 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596301 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596299 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596298 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596297 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596300 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596296 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596295 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596294 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596293 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596292 PRJNA550323 Mus musculus RNA-Seq
SRR9596291 PRJNA550323 Mus musculus RNA-Seq
SRR9596290 PRJNA550323 Mus musculus RNA-Seq
SRR9596289 PRJNA550323 Mus musculus RNA-Seq
SRR9596288 PRJNA550323 Mus musculus RNA-Seq
SRR9596287 PRJNA550323 Mus musculus RNA-Seq
SRR9596286 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596285 PRJNA550323 Rattus norvegicus Ribo-Seq
SRR9596284 PRJNA550323 Mus musculus RNA-Seq
SRR9596283 PRJNA550323 Mus musculus RNA-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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