Biever et al. 2020 (PRJNA550323)
General Details
Title | Monosomes actively translate synaptic mRNAs in neuronal processes |
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Organism | |
Number of Samples | 39 |
Release Date | 2019/06/24 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP211959 |
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ENA | SRP211959 |
GEO | |
BioProject | PRJNA550323 |
Publication
Title | |
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Authors | Biever A,Glock C,Tushev G,Ciirdaeva E,Dalmay T,Langer JD,Schuman EM |
Journal | Science (New York, N.Y.) |
Publication Date | 2020 Jan 31 |
Abstract | To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. |
PMC | |
PMID | 32001627 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR9596324 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596323 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596320 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596318 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596317 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596316 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596315 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596314 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596313 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596312 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596311 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596310 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596309 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596307 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596306 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596305 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596308 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596304 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596303 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596302 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596301 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596299 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596298 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596297 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596300 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596296 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596295 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596294 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596293 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596292 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596291 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596290 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596289 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596288 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596287 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596286 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596285 | PRJNA550323 | Rattus norvegicus | Ribo-Seq | ||||||||||
SRR9596284 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
SRR9596283 | PRJNA550323 | Mus musculus | RNA-Seq | ||||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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