Zhang et al. 2019 (PRJNA553160)

General Details

Title Ribosome profiling of GC7/vehicle-treated mouse primary B cells
Organism
Number of Samples 8
Release Date 2019/07/08 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP213616
ENA SRP213616
GEO
BioProject PRJNA553160

Publication

Title
Authors Zhang H,Alsaleh G,Feltham J,Sun Y,Napolitano G,Riffelmacher T,Charles P,Frau L,Hublitz P,Yu Z,Mohammed S,Ballabio A,Balabanov S,Mellor J,Simon AK
Journal Molecular cell
Publication Date 2019 Oct 3
Abstract Failure to make adaptive immune responses is a hallmark of aging. Reduced B cell function leads to poor vaccination efficacy and a high prevalence of infections in the elderly. Here we show that reduced autophagy is a central molecular mechanism underlying immune senescence. Autophagy levels are specifically reduced in mature lymphocytes, leading to compromised memory B cell responses in old individuals. Spermidine, an endogenous polyamine metabolite, induces autophagy in vivo and rejuvenates memory B cell responses. Mechanistically, spermidine post-translationally modifies the translation factor eIF5A, which is essential for the synthesis of the autophagy transcription factor TFEB. Spermidine is depleted in the elderly, leading to reduced TFEB expression and autophagy. Spermidine supplementation restored this pathway and improved the responses of old human B cells. Taken together, our results reveal an unexpected autophagy regulatory mechanism mediated by eIF5A at the translational level, which can be harnessed to reverse immune senescence in humans. Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
PMC PMC6863385
PMID 31474573
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR9651902 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651903 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651904 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651905 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651906 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651907 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651908 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
SRR9651909 PRJNA553160 Mus musculus primary splenic B cells Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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