Datasets

Title	Disome and trisome profiling reveal targets of ribosome quality control
Organism
Number of Samples	7
Release Date	2019/10/17 00:00
Sequencing Types
Protocol Details

Title
Authors	Meydan S,Guydosh NR
Journal	Molecular cell
Publication Date	2020 Aug 20
Abstract	The ribosome-associated protein quality control (RQC) system that resolves stalled translation events is activated when ribosomes collide and form disome, trisome, or higher-order complexes. However, it is unclear whether this system distinguishes collision complexes formed on defective mRNAs from those with functional roles on endogenous transcripts. Here, we performed disome and trisome footprint profiling in yeast and found collisions were enriched on diverse sequence motifs known to slow translation. When 60S recycling was inhibited, disomes accumulated at stop codons and could move into the 3' UTR to reinitiate translation. The ubiquitin ligase and RQC factor Hel2/ZNF598 generally recognized collisions but did not induce degradation of endogenous transcripts. However, loss of Hel2 triggered the integrated stress response, via phosphorylation of eIF2α, thus linking these pathways. Our results suggest that Hel2 has a role in sensing ribosome collisions on endogenous mRNAs, and such events may be important for cellular homeostasis. Published by Elsevier Inc.
PMC	PMC7484464
PMID	32615089
DOI

Meydan et al. 2020 (PRJNA578122)