Dai et al. 2019 (PRJNA579549)

Title	Ribosome profiling study of Miwi-null testes
Organism
Number of Samples	4
Release Date	2019/10/25 00:00
Sequencing Types
Protocol Details

GWIPS-viz	Trips-Viz

Title
Authors	Dai P,Wang X,Gou LT,Li ZT,Wen Z,Chen ZG,Hua MM,Zhong A,Wang L,Su H,Wan H,Qian K,Liao L,Li J,Tian B,Li D,Fu XD,Shi HJ,Zhou Y,Liu MF
Journal	Cell
Publication Date	2019 Dec 12
Abstract	Spermiogenesis is a highly orchestrated developmental process during which chromatin condensation decouples transcription from translation. Spermiogenic mRNAs are transcribed earlier and stored in a translationally inert state until needed for translation; however, it remains largely unclear how such repressed mRNAs become activated during spermiogenesis. We previously reported that the MIWI/piRNA machinery is responsible for mRNA elimination during late spermiogenesis in preparation for spermatozoa production. Here we unexpectedly discover that the same machinery is also responsible for activating translation of a subset of spermiogenic mRNAs to coordinate with morphological transformation into spermatozoa. Such action requires specific base-pairing interactions of piRNAs with target mRNAs in their 3' UTRs, which activates translation through coupling with cis-acting AU-rich elements to nucleate the formation of a MIWI/piRNA/eIF3f/HuR super-complex in a developmental stage-specific manner. These findings reveal a critical role of the piRNA system in translation activation, which we show is functionally required for spermatid development. Copyright © 2019 Elsevier Inc. All rights reserved.
PMC	PMC8139323
PMID	31835033
DOI

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