Nakazawa et al. 2020 (PRJNA594945)

General Details

Title Change in translation efficiency in mouse eyes at P0.5 by RNG140 knockout
Organism
Number of Samples 6
Release Date 2019/12/11 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP236878
ENA SRP236878
GEO GSE141842
BioProject PRJNA594945

Publication

Title
Authors Nakazawa K,Shichino Y,Iwasaki S,Shiina N
Journal The Journal of biological chemistry
Publication Date 2020 Oct 30
Abstract Regulation of gene expression at the translational level is key to determining cell fate and function. An RNA-binding protein, RNG140 (caprin2), plays a role in eye lens differentiation and has been reported to function in translational regulation. However, the mechanism and its role in eyes has remained unclear. Here, we show that RNG140 binds to the translation initiation factor eukaryotic initiation factor 3 (eIF3) and suppresses translation through mechanisms involving suppression of eIF3-dependent translation initiation. Comprehensive ribosome profiling revealed that overexpression of RNG140 in cultured Chinese hamster ovary cells reduces translation of long mRNAs, including those associated with cell proliferation. RNG140-mediated translational regulation also operates in the mouse eye, where RNG140 knockout increased the translation of long mRNAs. mRNAs involved in lens differentiation, such as crystallin mRNAs, are short and can escape translational inhibition by RNG140 and be translated in differentiating lenses. Thus, this study provides insights into the mechanistic basis of lens cell transition from proliferation to differentiation via RNG140-mediated translational regulation. © 2020 Nakazawa et al.
PMC PMC7606684
PMID 32839273
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR10669500 PRJNA594945 Mus musculus Ribo-Seq
SRR10669501 PRJNA594945 Mus musculus Ribo-Seq
SRR10669502 PRJNA594945 Mus musculus Ribo-Seq
SRR10669503 PRJNA594945 Mus musculus Ribo-Seq
SRR10669504 PRJNA594945 Mus musculus Ribo-Seq
SRR10669505 PRJNA594945 Mus musculus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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