Liu et al. 2021 (PRJNA597326)
General Details
| Title | Sulfur-dependent translational regulation via methylation multiplicity of 18S rRNA |
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| Organism | |
| Number of Samples | 12 |
| Release Date | 2019/12/23 00:00 |
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Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP238588 |
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| ENA | SRP238588 |
| GEO | |
| BioProject | PRJNA597326 |
Publication
| Title | |
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| Authors | Liu K,Santos DA,Hussmann JA,Wang Y,Sutter BM,Weissman JS,Tu BP |
| Journal | Cell reports |
| Publication Date | 2021 Mar 9 |
| Abstract | N 6 -methyladenosine (m 6 A) is a conserved ribonucleoside modification that regulates many facets of RNA metabolism. Using quantitative mass spectrometry, we find that the universally conserved tandem adenosines at the 3' end of 18S rRNA, thought to be constitutively di-methylated (m 6 2 A), are also mono-methylated (m 6 A). Although present at substoichiometric amounts, m 6 A at these positions increases significantly in response to sulfur starvation in yeast cells and mammalian cell lines. Combining yeast genetics and ribosome profiling, we provide evidence to suggest that m 6 A-bearing ribosomes carry out translation distinctly from m 6 2 A-bearing ribosomes, featuring a striking specificity for sulfur metabolism genes. Our work thus reveals methylation multiplicity as a mechanism to regulate translation. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved. |
| PMC | PMC8063911 |
| PMID | 33691096 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR10759495 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide |  |
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| SRR10759496 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759497 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759498 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759499 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759500 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759501 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759502 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759503 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759504 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759505 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| SRR10759506 | PRJNA597326 | Saccharomyces cerevisiae | Ribo-Seq | Cycloheximide | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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