Datasets

Title	Ribosome profiling under ABCE1 depletion to study aberrant translation termination
Organism
Number of Samples	12
Release Date	2020/01/08 00:00
Sequencing Types
Protocol Details

Title
Authors	Karousis ED,Gurzeler LA,Annibaldis G,Dreos R,Mühlemann O
Journal	Nature communications
Publication Date	2020 Aug 17
Abstract	Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway that is important for the elimination of faulty, and the regulation of normal, mRNAs. The molecular details of the early steps in NMD are not fully understood but previous work suggests that NMD activation occurs as a consequence of ribosome stalling at the termination codon (TC). To test this hypothesis, we established an in vitro translation-coupled toeprinting assay based on lysates from human cells that allows monitoring of ribosome occupancy at the TC of reporter mRNAs. In contrast to the prevailing NMD model, our in vitro system reveals similar ribosomal occupancy at the stop codons of NMD-sensitive and NMD-insensitive reporter mRNAs. Moreover, ribosome profiling reveals a similar density of ribosomes at the TC of endogenous NMD-sensitive and NMD-insensitive mRNAs in vivo. Together, these data show that NMD activation is not accompanied by stable stalling of ribosomes at TCs.
PMC	PMC7431590
PMID	32807779
DOI

Karousis et al. 2020 (PRJNA599943)