Shah et al. 2020 (PRJNA600029)

General Details

Title FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism (Steady-state ribosome profiling)
Organism
Number of Samples 10
Release Date 2020/01/08 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP240703
ENA SRP240703
GEO GSE143330
BioProject PRJNA600029

Publication

Title
Authors Shah S,Molinaro G,Liu B,Wang R,Huber KM,Richter JD
Journal Cell reports
Publication Date 2020 Mar 31
Abstract Silencing of FMR1 and loss of its gene product, FMRP, results in fragile X syndrome (FXS). FMRP binds brain mRNAs and inhibits polypeptide elongation. Using ribosome profiling of the hippocampus, we find that ribosome footprint levels in Fmr1-deficient tissue mostly reflect changes in RNA abundance. Profiling over a time course of ribosome runoff in wild-type tissue reveals a wide range of ribosome translocation rates; on many mRNAs, the ribosomes are stalled. Sucrose gradient ultracentrifugation of hippocampal slices after ribosome runoff reveals that FMRP co-sediments with stalled ribosomes, and its loss results in decline of ribosome stalling on specific mRNAs. One such mRNA encodes SETD2, a lysine methyltransferase that catalyzes H3K36me3. Chromatin immunoprecipitation sequencing (ChIP-seq) demonstrates that loss of FMRP alters the deployment of this histone mark. H3K36me3 is associated with alternative pre-RNA processing, which we find occurs in an FMRP-dependent manner on transcripts linked to neural function and autism spectrum disorders. Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
PMC PMC7179797
PMID 32234480
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR10919493 PRJNA600029 Mus musculus Ribo-Seq
SRR10919494 PRJNA600029 Mus musculus Ribo-Seq
SRR10919495 PRJNA600029 Mus musculus Ribo-Seq
SRR10919496 PRJNA600029 Mus musculus Ribo-Seq
SRR10919497 PRJNA600029 Mus musculus Ribo-Seq
SRR10919498 PRJNA600029 Mus musculus Ribo-Seq
SRR10919499 PRJNA600029 Mus musculus Ribo-Seq
SRR10919500 PRJNA600029 Mus musculus Ribo-Seq
SRR10919501 PRJNA600029 Mus musculus Ribo-Seq
SRR10919502 PRJNA600029 Mus musculus Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

ⓘ For more Information on the columns shown here see: About