Shah et al. 2020 (PRJNA600029)
General Details
Title | FMRP Control of Ribosome Translocation Promotes Chromatin Modifications and Alternative Splicing of Neuronal Genes Linked to Autism (Steady-state ribosome profiling) |
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Organism | |
Number of Samples | 10 |
Release Date | 2020/01/08 00:00 |
Sequencing Types | |
Protocol Details |
Study Links
GWIPS-viz | Trips-Viz |
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Repository Details
SRA | SRP240703 |
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ENA | SRP240703 |
GEO | GSE143330 |
BioProject | PRJNA600029 |
Publication
Title | |
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Authors | Shah S,Molinaro G,Liu B,Wang R,Huber KM,Richter JD |
Journal | Cell reports |
Publication Date | 2020 Mar 31 |
Abstract | Silencing of FMR1 and loss of its gene product, FMRP, results in fragile X syndrome (FXS). FMRP binds brain mRNAs and inhibits polypeptide elongation. Using ribosome profiling of the hippocampus, we find that ribosome footprint levels in Fmr1-deficient tissue mostly reflect changes in RNA abundance. Profiling over a time course of ribosome runoff in wild-type tissue reveals a wide range of ribosome translocation rates; on many mRNAs, the ribosomes are stalled. Sucrose gradient ultracentrifugation of hippocampal slices after ribosome runoff reveals that FMRP co-sediments with stalled ribosomes, and its loss results in decline of ribosome stalling on specific mRNAs. One such mRNA encodes SETD2, a lysine methyltransferase that catalyzes H3K36me3. Chromatin immunoprecipitation sequencing (ChIP-seq) demonstrates that loss of FMRP alters the deployment of this histone mark. H3K36me3 is associated with alternative pre-RNA processing, which we find occurs in an FMRP-dependent manner on transcripts linked to neural function and autism spectrum disorders. Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved. |
PMC | PMC7179797 |
PMID | 32234480 |
DOI |
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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SRR10919493 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919494 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919495 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919496 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919497 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919498 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919499 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919500 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919501 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
SRR10919502 | PRJNA600029 | Mus musculus | Ribo-Seq | ||||||||||
Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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