Datasets

Title	A PRC2-independent function for EZH2 in regulating rRNA 2′-O methylation and IRES-dependent translation
Organism
Number of Samples	9
Release Date	2020/01/21 00:00
Sequencing Types
Protocol Details

Title
Authors	Yi Y,Li Y,Meng Q,Li Q,Li F,Lu B,Shen J,Fazli L,Zhao D,Li C,Jiang W,Wang R,Liu Q,Szczepanski A,Li Q,Qin W,Weiner AB,Lotan TL,Ji Z,Kalantry S,Wang L,Schaeffer EM,Niu H,Dong X,Zhao W,Chen K,Cao Q
Journal	Nature cell biology
Publication Date	2021 Apr
Abstract	Dysregulated translation is a common feature of cancer. Uncovering its governing factors and underlying mechanism are important for cancer therapy. Here, we report that enhancer of zeste homologue 2 (EZH2), previously known as a transcription repressor and lysine methyltransferase, can directly interact with fibrillarin (FBL) to exert its role in translational regulation. We demonstrate that EZH2 enhances rRNA 2'-O methylation via its direct interaction with FBL. Mechanistically, EZH2 strengthens the FBL-NOP56 interaction and facilitates the assembly of box C/D small nucleolar ribonucleoprotein. Strikingly, EZH2 deficiency impairs the translation process globally and reduces internal ribosome entry site (IRES)-dependent translation initiation in cancer cells. Our findings reveal a previously unrecognized role of EZH2 in cancer-related translational regulation.
PMC	PMC8162121
PMID	33795875
DOI

Yi et al. 2020 (PRJNA602407)