Muller et al. 2020 (PRJNA608916)

General Details

Title Microbes modulate sympathetic neurons via a gut-brain circuit
Organism
Number of Samples 24
Release Date 2020/02/26 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP250834
ENA SRP250834
GEO GSE145986
BioProject PRJNA608916

Publication

Title
Authors Muller PA,Schneeberger M,Matheis F,Wang P,Kerner Z,Ilanges A,Pellegrino K,Del Mármol J,Castro TBR,Furuichi M,Perkins M,Han W,Rao A,Pickard AJ,Cross JR,Honda K,de Araujo I,Mucida D
Journal Nature
Publication Date 2020 Jul
Abstract Connections between the gut and brain monitor the intestinal tissue and its microbial and dietary content 1 , regulating both physiological intestinal functions such as nutrient absorption and motility 2,3 , and brain-wired feeding behaviour 2 . It is therefore plausible that circuits exist to detect gut microorganisms and relay this information to areas of the central nervous system that, in turn, regulate gut physiology 4 . Here we characterize the influence of the microbiota on enteric-associated neurons by combining gnotobiotic mouse models with transcriptomics, circuit-tracing methods and functional manipulations. We find that the gut microbiome modulates gut-extrinsic sympathetic neurons: microbiota depletion leads to increased expression of the neuronal transcription factor cFos, and colonization of germ-free mice with bacteria that produce short-chain fatty acids suppresses cFos expression in the gut sympathetic ganglia. Chemogenetic manipulations, translational profiling and anterograde tracing identify a subset of distal intestine-projecting vagal neurons that are positioned to have an afferent role in microbiota-mediated modulation of gut sympathetic neurons. Retrograde polysynaptic neuronal tracing from the intestinal wall identifies brainstem sensory nuclei that are activated during microbial depletion, as well as efferent sympathetic premotor glutamatergic neurons that regulate gastrointestinal transit. These results reveal microbiota-dependent control of gut-extrinsic sympathetic activation through a gut-brain circuit.
PMC PMC7367767
PMID 32641826
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR11186577 PRJNA608916 Mus musculus RIP
SRR11186578 PRJNA608916 Mus musculus RIP
SRR11186579 PRJNA608916 Mus musculus RIP
SRR11186580 PRJNA608916 Mus musculus RIP
SRR11186581 PRJNA608916 Mus musculus RIP
SRR11186582 PRJNA608916 Mus musculus RIP
SRR11186583 PRJNA608916 Mus musculus RIP
SRR11186584 PRJNA608916 Mus musculus RIP
SRR11186585 PRJNA608916 Mus musculus RIP
SRR11186586 PRJNA608916 Mus musculus RIP
SRR11186587 PRJNA608916 Mus musculus RIP
SRR11186588 PRJNA608916 Mus musculus RIP
SRR11186589 PRJNA608916 Mus musculus RIP
SRR11186590 PRJNA608916 Mus musculus RIP
SRR11186591 PRJNA608916 Mus musculus RIP
SRR11186592 PRJNA608916 Mus musculus RIP
SRR11186593 PRJNA608916 Mus musculus
SRR11186594 PRJNA608916 Mus musculus
SRR11186595 PRJNA608916 Mus musculus
SRR11186596 PRJNA608916 Mus musculus
SRR11186597 PRJNA608916 Mus musculus
SRR11186598 PRJNA608916 Mus musculus
SRR11186599 PRJNA608916 Mus musculus
SRR11186600 PRJNA608916 Mus musculus
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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