Datasets

Title	Ribosome profiling analysis of aging human skeletal muscle (Ribo-Seq)
Organism
Number of Samples	5
Release Date	2020/06/16 00:00
Sequencing Types
Protocol Details

Title
Authors	Tharakan R,Ubaida-Mohien C,Piao Y,Gorospe M,Ferrucci L
Journal	RNA biology
Publication Date	2021 Nov
Abstract	With advancing age, human muscle loses strength and function, but the molecular causes of these losses are unknown. Skeletal muscle shows an age-dependent decline in the levels of different proteins, but whether such decline is associated with reduced translation has not been studied. To address this gap of knowledge, we used the technique of ribosome profiling to study translation in muscle from middle-aged and old individuals. Using ribosome occupancy as a measure of translation status, several mRNAs showed differential translation with age. Older age was associated with lower translation of myosin and titin isoforms and more broadly with the translation of proteins involved in oxidative phosphorylation encoded by the mitochondrial genome. Based on our findings, we propose that mitochondrial proteins are less translated in old skeletal muscle.
PMC	PMC8583053
PMID	33472542
DOI

Tharakan et al. 2021 (PRJNA639716)