Datasets

Title	Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs
Organism
Number of Samples	5
Release Date	2020/07/29 00:00
Sequencing Types
Protocol Details

Title
Authors	Sun YH, Wang RH, Du K, Zhu J, Zheng J, Xie LH, Pereira AA, Zhang C, Ricci EP, Li XZ
Journal	Nature communications
Publication Date	2021 Oct 13
Abstract	PIWI-interacting small RNAs (piRNAs) protect the germline genome and are essential for fertility. piRNAs originate from transposable element (TE) RNAs, long non-coding RNAs, or 3´ untranslated regions (3´UTRs) of protein-coding messenger genes, with the last being the least characterized of the three piRNA classes. Here, we demonstrate that the precursors of 3´UTR piRNAs are full-length mRNAs and that post-termination 80S ribosomes guide piRNA production on 3´UTRs in mice and chickens. At the pachytene stage, when other co-translational RNA surveillance pathways are sequestered, piRNA biogenesis degrades mRNAs right after pioneer rounds of translation and fine-tunes protein production from mRNAs. Although 3´UTR piRNA precursor mRNAs code for distinct proteins in mice and chickens, they all harbor embedded TEs and produce piRNAs that cleave TEs. Altogether, we discover a function of the piRNA pathway in fine-tuning protein production and reveal a conserved piRNA biogenesis mechanism that recognizes translating RNAs in amniotes. © 2021. The Author(s).
PMC	PMC8514520
PMID	34645830
DOI

Run Accession	Study Accession	Scientific Name	Cell Line	Library Type	Treatment	GWIPS-viz	Trips-Viz	Reads	BAM	BigWig (F)	BigWig (R)
SRR12343757	PRJNA649449	Mus musculus	0.0	Ribo-Seq	0.0
SRR12343758	PRJNA649449	Mus musculus	0.0	Ribo-Seq	0.0
SRR16492701	PRJNA649449	Mus musculus	0.0	RNA-Seq	0.0
SRR16492702	PRJNA649449	Mus musculus	0.0	Ribo-Seq	0.0
SRR16492703	PRJNA649449	Mus musculus	0.0	Ribo-Seq	0.0
Run Accession	Study Accession	Scientific Name	Cell Line	Library Type	Treatment	GWIPS-viz	Trips-Viz	Reads	BAM	BigWig (F)	BigWig (R)

Sun et al. 2021 (PRJNA649449)