Datasets

Title	RNA-seq and Ribo-seq analysis of the effects of METTL3/14 depletion on gene expression during the interferon response in Huh7 cells
Organism
Number of Samples	8
Release Date	2020/07/30 00:00
Sequencing Types
Protocol Details

Title
Authors	McFadden MJ,McIntyre ABR,Mourelatos H,Abell NS,Gokhale NS,Ipas H,Xhemalçe B,Mason CE,Horner SM
Journal	Cell reports
Publication Date	2021 Mar 2
Abstract	Type I interferons (IFNs) induce hundreds of IFN-stimulated genes (ISGs) in response to viral infection. Induction of these ISGs must be regulated for an efficient and controlled antiviral response, but post-transcriptional controls of these genes have not been well defined. Here, we identify a role for the RNA base modification N6-methyladenosine (m 6 A) in the regulation of ISGs. Using ribosome profiling and quantitative mass spectrometry, coupled with m 6 A-immunoprecipitation and sequencing, we identify a subset of ISGs, including IFITM1, whose translation is enhanced by m 6 A and the m 6 A methyltransferase proteins METTL3 and METTL14. We further determine that the m 6 A reader YTHDF1 increases the expression of IFITM1 in an m 6 A-binding-dependent manner. Importantly, we find that the m 6 A methyltransferase complex promotes the antiviral activity of type I IFN. Thus, these studies identify m 6 A as having a role in post-transcriptional control of ISG translation during the type I IFN response for antiviral restriction. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
PMC	PMC7981787
PMID	33657363
DOI

McFadden et al. 2021 (PRJNA649776)