Jobava et al. 2021 (PRJNA661558)
General Details
| Title | Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress |
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| Organism | |
| Number of Samples | 18 |
| Release Date | 2020/09/04 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP280144 |
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| ENA | SRP280144 |
| GEO | |
| BioProject | PRJNA661558 |
Publication
| Title | |
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| Authors | Jobava R,Mao Y,Guan BJ,Hu D,Krokowski D,Chen CW,Shu XE,Chukwurah E,Wu J,Gao Z,Zagore LL,Merrick WC,Trifunovic A,Hsieh AC,Valadkhan S,Zhang Y,Qi X,Jankowsky E,Topisirovic I,Licatalosi DD,Qian SB,Hatzoglou M |
| Journal | Molecular cell |
| Publication Date | 2021 Oct 21 |
| Abstract | To survive, mammalian cells must adapt to environmental challenges. While the cellular response to mild stress has been widely studied, how cells respond to severe stress remains unclear. We show here that under severe hyperosmotic stress, cells enter a transient hibernation-like state in anticipation of recovery. We demonstrate this adaptive pausing response (APR) is a coordinated cellular response that limits ATP supply and consumption through mitochondrial fragmentation and widespread pausing of mRNA translation. This pausing is accomplished by ribosome stalling at translation initiation codons, which keeps mRNAs poised to resume translation upon recovery. We further show that recovery from severe stress involves ISR (integrated stress response) signaling that permits cell cycle progression, resumption of growth, and reversal of mitochondria fragmentation. Our findings indicate that cells can respond to severe stress via a hibernation-like mechanism that preserves vital elements of cellular function under harsh environmental conditions. Copyright © 2021 Elsevier Inc. All rights reserved. |
| PMC | PMC8559772 |
| PMID | 34686314 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR12594201 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq |  |
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| SRR12594202 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594203 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594204 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594205 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594206 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594207 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594208 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594209 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594210 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594211 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594212 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594213 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594214 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594215 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594216 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594217 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| SRR12594218 | PRJNA661558 | Mus musculus | MEFs (mouse embryonic fibroblasts) | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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