Datasets

Title	Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome
Organism
Number of Samples	12
Release Date	2021/02/24 00:00
Sequencing Types
Protocol Details

Title
Authors	Bhatt PR,Scaiola A,Loughran G,Leibundgut M,Kratzel A,Meurs R,Dreos R,O'Connor KM,McMillan A,Bode JW,Thiel V,Gatfield D,Atkins JF,Ban N
Journal	Science (New York, N.Y.)
Publication Date	2021 Jun 18
Abstract	Programmed ribosomal frameshifting is a key event during translation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome that allows synthesis of the viral RNA-dependent RNA polymerase and downstream proteins. Here, we present the cryo-electron microscopy structure of a translating mammalian ribosome primed for frameshifting on the viral RNA. The viral RNA adopts a pseudoknot structure that lodges at the entry to the ribosomal messenger RNA (mRNA) channel to generate tension in the mRNA and promote frameshifting, whereas the nascent viral polyprotein forms distinct interactions with the ribosomal tunnel. Biochemical experiments validate the structural observations and reveal mechanistic and regulatory features that influence frameshifting efficiency. Finally, we compare compounds previously shown to reduce frameshifting with respect to their ability to inhibit SARS-CoV-2 replication, establishing coronavirus frameshifting as a target for antiviral intervention. Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMC	PMC8168617
PMID	34029205
DOI

Bhatt et al. 2021 (PRJNA704627)