Bohlen et al. 2021 (PRJNA714824)

General Details

Title Phosphorylation of Ribosomal Protein S6 differentially affects mRNA translation based on ORF length
Organism
Number of Samples 7
Release Date 2021/03/16 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP310845
ENA SRP310845
GEO GSE168977
BioProject PRJNA714824

Publication

Title
Authors Bohlen J, Roiuk M, Teleman AA
Journal Nucleic acids research
Publication Date 2021 Dec 16
Abstract Phosphorylation of Ribosomal Protein S6 (RPS6) was the first post-translational modification of the ribosome to be identified and is a commonly-used readout for mTORC1 activity. Although the cellular and organismal functions of RPS6 phosphorylation are known, the molecular consequences of RPS6 phosphorylation on translation are less well understood. Here we use selective ribosome footprinting to analyze the location of ribosomes containing phosphorylated RPS6 on endogenous mRNAs in cells. We find that RPS6 becomes progressively dephosphorylated on ribosomes as they translate an mRNA. As a consequence, average RPS6 phosphorylation is higher on mRNAs with short coding sequences (CDSs) compared to mRNAs with long CDSs. We test whether RPS6 phosphorylation differentially affects mRNA translation based on CDS length by genetic removal of RPS6 phosphorylation. We find that RPS6 phosphorylation promotes translation of mRNAs with short CDSs more strongly than mRNAs with long CDSs. Interestingly, RPS6 phosphorylation does not promote translation of mRNAs with 5' TOP motifs despite their short CDS lengths, suggesting they are translated via a different mode. In sum this provides a dynamic view of RPS6 phosphorylation on ribosomes as they translate mRNAs and the functional consequence on translation. © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC8682771
PMID 34871442
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR13976755 PRJNA714824 Homo sapiens HeLa Ribo-Seq 0.0
SRR13976756 PRJNA714824 Homo sapiens HeLa Ribo-Seq 0.0
SRR13976757 PRJNA714824 Mus musculus 0.0 Ribo-Seq 0.0
SRR13976758 PRJNA714824 Mus musculus 0.0 Ribo-Seq 0.0
SRR13976759 PRJNA714824 Mus musculus 0.0 Ribo-Seq 0.0
SRR13976760 PRJNA714824 Mus musculus 0.0 RNA-Seq 0.0
SRR13976761 PRJNA714824 Mus musculus 0.0 RNA-Seq 0.0
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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