Zhang et al. 2021 (PRJNA717246)
General Details
| Title | Profiling and functional characterization of maternal mRNA translation in mouse maternal-to-zygotic transition |
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| Organism | |
| Number of Samples | 14 |
| Release Date | 2021/03/25 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP312148 |
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| ENA | SRP312148 |
| GEO | |
| BioProject | PRJNA717246 |
Publication
| Title | |
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| Authors | Zhang C,Wang M,Li Y,Zhang Y |
| Journal | Science advances |
| Publication Date | 2022 Feb 4 |
| Abstract | Translational regulation plays an important role in gene expression and function. Although the transcriptional dynamics of mouse preimplantation embryos have been well characterized, the global mRNA translation landscape and the master regulators of zygotic genome activation (ZGA) remain unknown. Here, by developing and applying a low-input ribosome profiling (LiRibo-seq) technique, we profiled the mRNA translation landscape in mouse preimplantation embryos and revealed the translational dynamics during mouse preimplantation development. We identified a marked translational transition from MII oocytes to zygotes and demonstrated that active translation of maternal mRNAs is essential for maternal-to-zygotic transition (MZT). We further showed that two maternal factors, Smarcd2 and Cyclin T2, whose translation is activated in zygotes, are required for chromatin reprogramming and ZGA, respectively. Our study thus not only filled in a knowledge gap on translational regulation during mammalian preimplantation development but also revealed insights into the critical function of maternal mRNA translation in MZT. |
| PMC | PMC8809684 |
| PMID | 35108058 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR15960262 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated |  |
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| SRR15960263 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960264 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960265 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960266 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960267 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960268 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960269 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960270 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960257 | PRJNA717246 | Mus musculus | mESC | Ribo-Seq | untreated | |
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| SRR15960258 | PRJNA717246 | Mus musculus | mESC | Ribo-Seq | untreated | |
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| SRR15960259 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960260 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| SRR15960261 | PRJNA717246 | Mus musculus | Ribo-Seq | untreated | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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