Datasets

Title	Nucleolin HITS-CLIP, RNA-Seq and Ribo-Seq upon inhibition of 5'-tRFCys
Organism
Number of Samples	8
Release Date	2021/04/26 00:00
Sequencing Types
Protocol Details

Title
Authors	Liu X,Mei W,Padmanaban V,Alwaseem H,Molina H,Passarelli MC,Tavora B,Tavazoie SF
Journal	Molecular cell
Publication Date	2022 Jul 21
Abstract	Stress-induced cleavage of transfer RNAs (tRNAs) into tRNA-derived fragments (tRFs) occurs across organisms from yeast to humans; yet, its mechanistic underpinnings and pathological consequences remain poorly defined. Small RNA profiling revealed increased abundance of a cysteine tRNA fragment (5'-tRF Cys ) during breast cancer metastatic progression. 5'-tRF Cys was required for efficient breast cancer metastatic lung colonization and cancer cell survival. We identified Nucleolin as the direct binding partner of 5'-tRF Cys . 5'-tRF Cys promoted the oligomerization of Nucleolin and its bound metabolic transcripts Mthfd1l and Pafah1b1 into a higher-order transcript stabilizing ribonucleoprotein complex, which protected these transcripts from exonucleolytic degradation. Consistent with this, Mthfd1l and Pafah1b1 mediated pro-metastatic and metabolic effects downstream of 5'-tRF Cys -impacting folate, one-carbon, and phosphatidylcholine metabolism. Our findings reveal that a tRF can promote oligomerization of an RNA-binding protein into a transcript stabilizing ribonucleoprotein complex, thereby driving specific metabolic pathways underlying cancer progression. Copyright © 2022 Elsevier Inc. All rights reserved.
PMC	PMC9444141
PMID	35654044
DOI

Liu et al. 2022 (PRJNA725264)