Teyssonniere et al. 2024 (PRJNA726548)

General Details

Title Translation variation across genetic backgrounds reveals a post-transcriptional buffering signature in yeast
Organism
Number of Samples 16
Release Date 2021/04/30 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP318024
ENA SRP318024
GEO GSE173654
BioProject PRJNA726548

Publication

Title
Authors Teyssonniere EM,Shichino Y,Mito M,Friedrich A,Iwasaki S,Schacherer J
Journal Nucleic acids research
Publication Date 2024 Mar 21
Abstract Gene expression is known to vary among individuals, and this variability can impact the phenotypic diversity observed in natural populations. While the transcriptome and proteome have been extensively studied, little is known about the translation process itself. Here, we therefore performed ribosome and transcriptomic profiling on a genetically and ecologically diverse set of natural isolates of the Saccharomyces cerevisiae yeast. Interestingly, we found that the Euclidean distances between each profile and the expression fold changes in each pairwise isolate comparison were higher at the transcriptomic level. This observation clearly indicates that the transcriptional variation observed in the different isolates is buffered through a phenomenon known as post-transcriptional buffering at the translation level. Furthermore, this phenomenon seemed to have a specific signature by preferentially affecting essential genes as well as genes involved in complex-forming proteins, and low transcribed genes. We also explored the translation of the S. cerevisiae pangenome and found that the accessory genes related to introgression events displayed similar transcription and translation levels as the core genome. By contrast, genes acquired through horizontal gene transfer events tended to be less efficiently translated. Together, our results highlight both the extent and signature of the post-transcriptional buffering. © The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.
PMC PMC10954453
PMID 38261993
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR26680426 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680427 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680428 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680429 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680430 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680431 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680432 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680433 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680434 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680435 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680436 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680437 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680438 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680439 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680440 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
SRR26680441 PRJNA726548 Saccharomyces cerevisiae yeast cells Ribo-Seq
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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