Shu et al. 2021 (PRJNA750456)

General Details

Title Dynamic O-GlcNAcylation of eIF3a controls translation reinitiation during integrated stress response
Organism
Number of Samples 20
Release Date 2021/07/28 00:00
Sequencing Types
Protocol Details

Study Links

Repository Details

SRA SRP330265
ENA SRP330265
GEO
BioProject PRJNA750456

Publication

Title
Authors Shu XE,Mao Y,Jia L,Qian SB
Journal Nature chemical biology
Publication Date 2022 Feb
Abstract In eukaryotic cells, many messenger RNAs (mRNAs) possess upstream open reading frames (uORFs) in addition to the main coding region. After uORF translation, the ribosome could either recycle at the stop codon or resume scanning for downstream start codons in a process known as reinitiation. Accumulating evidence suggests that some initiation factors, including eukaryotic initiation factor 3 (eIF3), linger on the early elongating ribosome, forming an eIF3-80S complex. Very little is known about how eIF3 is carried along with the 80S during elongation and whether the eIF3-80S association is subject to regulation. Here, we report that eIF3a undergoes dynamic O-linked N-acetylglucosamine (O-GlcNAc) modification in response to nutrient starvation. Stress-induced de-O-GlcNAcylation promotes eIF3 retention on the elongating ribosome and facilitates activating transcription factor 4 (ATF4) reinitiation. Eliminating the modification site from eIF3a via CRISPR genome editing induces ATF4 reinitiation even under the nutrient-rich condition. Our findings illustrate a mechanism in balancing ribosome recycling and reinitiation, thereby linking the nutrient stress response and translational reprogramming. © 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.
PMC PMC8810738
PMID 34887587
DOI
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)
SRR15283612 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283613 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283614 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283615 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283616 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283617 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283618 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283619 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283620 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283621 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283622 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283623 PRJNA750456 Mus musculus Mouse embryonic fibroblasts Ribo-Seq
SRR15283624 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283625 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283626 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283627 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283628 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283629 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283630 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
SRR15283631 PRJNA750456 Mus musculus Mouse embryonic fibroblasts LSU Frozen
Run Accession Study Accession Scientific Name Cell Line Library Type Treatment GWIPS-viz Trips-Viz Reads BAM BigWig (F) BigWig (R)

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