Martin et al. 2022 (PRJNA757962)
General Details
| Title | Oligodendrocyte differentiation alters tRNA modifications and codon-dependent mRNA decay [RiboSeq] |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2021/08/26 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP334193 |
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| ENA | SRP334193 |
| GEO | GSE182810 |
| BioProject | PRJNA757962 |
Publication
| Title | |
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| Authors | Martin S, Allan KC, Pinkard O, Sweet T, Tesar PJ, Coller J |
| Journal | Nature communications |
| Publication Date | 2022 Aug 25 |
| Abstract | Oligodendrocytes are specialized cells that confer neuronal myelination in the central nervous system. Leukodystrophies associated with oligodendrocyte deficits and hypomyelination are known to result when a number of tRNA metabolism genes are mutated. Thus, for unknown reasons, oligodendrocytes may be hypersensitive to perturbations in tRNA biology. In this study, we survey the tRNA transcriptome in the murine oligodendrocyte cell lineage and find that specific tRNAs are hypomodified in oligodendrocytes within or near the anticodon compared to oligodendrocyte progenitor cells (OPCs). This hypomodified state may be the result of differential expression of key modification enzymes during oligodendrocyte differentiation. Moreover, we observe a concomitant relationship between tRNA hypomodification and tRNA decoding potential; observing oligodendrocyte specific alterations in codon optimality-mediated mRNA decay and ribosome transit. Our results reveal that oligodendrocytes naturally maintain a delicate, hypersensitized tRNA/mRNA axis. We suggest this axis is a potential mediator of pathology in leukodystrophies and white matter disease when further insult to tRNA metabolism is introduced. © 2022. The Author(s). |
| PMC | PMC9411196 |
| PMID | 36008413 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR15618737 | PRJNA757962 | Mus musculus | OPC | Ribo-Seq | Frozen |  |
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| SRR15618738 | PRJNA757962 | Mus musculus | OPC | Ribo-Seq | Frozen | |
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| SRR15618741 | PRJNA757962 | Mus musculus | OL | Ribo-Seq | Frozen | |
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| SRR15618742 | PRJNA757962 | Mus musculus | OL | Ribo-Seq | Frozen | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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