Wu et al. 2022 (PRJNA763584)
General Details
| Title | TRL-1 undergoes phase separation to regulate the reproduction and longevity tradeoff |
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| Organism | |
| Number of Samples | 30 |
| Release Date | 2021/09/15 00:00 |
| Sequencing Types | |
| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP337255 |
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| ENA | SRP337255 |
| GEO | GSE184209 |
| BioProject | PRJNA763584 |
Publication
| Title | |
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| Authors | Wu D,Wang Z,Huang J,Huang L,Zhang S,Zhao R,Li W,Chen D,Ou G |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Publication Date | 2022 May 3 |
| Abstract | The antagonistic pleiotropy theory of aging proposes that genes enhancing fitness in early life limit the lifespan, but the molecular evidence remains underexplored. By profiling translatome changes in Caenorhabditis elegans during starvation recovery, we find that an open reading frame (ORF) trl-1 “hidden” within an annotated pseudogene significantly translates upon refeeding. trl-1 mutant animals increase brood sizes but shorten the lifespan and specifically impair germline deficiency–induced longevity. The loss of trl-1 abnormally up-regulates the translation of vitellogenin that produces copious yolk to provision eggs, whereas vitellogenin overexpression is known to reduce the lifespan. We show that the TRL-1 protein undergoes liquid–liquid phase separation (LLPS), through which TRL-1 granules recruit vitellogenin messenger RNA and inhibit its translation. These results indicate that trl-1 functions as an antagonistic pleiotropic gene to regulate the reproduction–longevity tradeoff by optimizing nutrient production for the next generation. |
| PMC | PMC9170148 |
| PMID | 35482917 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR15906252 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq |  |
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| SRR15906253 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906254 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906255 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906256 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906257 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906258 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906259 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906260 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906261 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906272 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906273 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906274 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906275 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906276 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906277 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906278 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906279 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906280 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906281 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906292 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906293 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906294 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906295 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906296 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906297 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906298 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906299 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906300 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| SRR15906301 | PRJNA763584 | Caenorhabditis elegans | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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