Chadani et al. 2021 (PRJNA764085)
General Details
| Title | Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation |
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| Organism | |
| Number of Samples | 4 |
| Release Date | 2021/09/17 00:00 |
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| Protocol Details |
Study Links
| GWIPS-viz | Trips-Viz |
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Repository Details
| SRA | SRP337604 |
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| ENA | SRP337604 |
| GEO | GSE184331 |
| BioProject | PRJNA764085 |
Publication
| Title | |
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| Authors | Chadani Y,Sugata N,Niwa T,Ito Y,Iwasaki S,Taguchi H |
| Journal | The EMBO journal |
| Publication Date | 2021 Dec 1 |
| Abstract | Continuous translation elongation, irrespective of amino acid sequences, is a prerequisite for living organisms to produce their proteomes. However, nascent polypeptide products bear an inherent risk of elongation abortion. For example, negatively charged sequences with occasional intermittent prolines, termed intrinsic ribosome destabilization (IRD) sequences, weaken the translating ribosomal complex, causing certain nascent chain sequences to prematurely terminate translation. Here, we show that most potential IRD sequences in the middle of open reading frames remain cryptic and do not interrupt translation, due to two features of the nascent polypeptide. Firstly, the nascent polypeptide itself spans the exit tunnel, and secondly, its bulky amino acid residues occupy the tunnel entrance region, thereby serving as a bridge and protecting the large and small ribosomal subunits from dissociation. Thus, nascent polypeptide products have an inbuilt ability to ensure elongation continuity. © 2021 The Authors. |
| PMC | PMC8634131 |
| PMID | 34672004 |
| DOI |
| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) | ||
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| SRR15929424 | PRJNA764085 | Escherichia coli | Ribo-Seq |  |
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| SRR15929425 | PRJNA764085 | Escherichia coli | Ribo-Seq | |
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| SRR15929426 | PRJNA764085 | Escherichia coli | Ribo-Seq | |
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| SRR15929427 | PRJNA764085 | Escherichia coli | Ribo-Seq | |
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| Run Accession | Study Accession | Scientific Name | Cell Line | Library Type | Treatment | GWIPS-viz | Trips-Viz | Reads | BAM | BigWig (F) | BigWig (R) |
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