Datasets

Title	A Single-copy Knock In Translating Ribosome ImmunoPrecipitation (SKI TRIP) tool kit for tissue specific profiling of actively translated mRNAs in C. elegans.
Organism
Number of Samples	21
Release Date	2022/01/28 00:00
Sequencing Types
Protocol Details

Title
Authors	Wester LE,Lanjuin A,Bruckisch EHW,Perez-Matos MC,Stine PG,Heintz C,Denzel MS,Mair WB
Journal	Cell reports methods
Publication Date	2023 Mar 27
Abstract	Here, we introduce a single-copy knockin translating ribosome immunoprecipitation (SKI TRIP) toolkit, a collection of Caenorhabditis elegans strains engineered by CRISPR in which tissue-specific expression of FLAG-tagged ribosomal subunit protein RPL-22 is driven by cassettes present in single copy from defined sites in the genome. Through in-depth characterization of the effects of the FLAG tag in animals in which endogenous RPL-22 has been tagged, we show that it incorporates into actively translating ribosomes and efficiently and cleanly pulls down cell-type-specific transcripts. Importantly, the presence of the tag does not impact overall mRNA translation, create bias in transcript use, or cause changes to fitness of the animal. We propose SKI TRIP use for the study of tissue-specific differences in translation and for investigating processes that are acutely sensitive to changes in translation like development or aging. © 2023 The Author(s).
PMC	PMC10088236
PMID	37056370
DOI

Wester et al. 2022 (PRJNA801467)