Datasets

Title	Stress-induced transcriptional readthrough into neighboring genes is linked to intron retention
Organism
Number of Samples	10
Release Date	2022/02/27 00:00
Sequencing Types
Protocol Details

Title
Authors	Hadar S, Meller A, Saida N, Shalgi R
Journal	iScience
Publication Date	2022 Dec 22
Abstract	Exposure to certain stresses leads to readthrough transcription. Using polyA-selected RNA-seq in mouse fibroblasts subjected to heat shock, oxidative, or osmotic stress, we found that readthrough transcription can proceed into proximal downstream genes, in a phenomenon previously termed 'read-in.' We found that read-in genes share distinctive genomic characteristics; they are GC-rich and extremely short , with genomic features conserved in human. Using ribosome profiling, we found that read-in genes show significantly reduced translation. Strikingly, read-in genes demonstrate marked intron retention, mostly in their first introns, which could not be explained solely by their short introns and GC-richness, features often associated with intron retention. Finally, we revealed H3K36me3 enrichment upstream to read-in genes. Moreover, demarcation of exon-intron junctions by H3K36me3 was absent in read-in first introns. Our data portray a relationship between read-in and intron retention, suggesting they may have co-evolved to facilitate reduced translation of read-in genes during stress. © 2022 The Authors.
PMC	PMC9732411
PMID	36505935
DOI

Hadar et al. 2022 (PRJNA810853)